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Effects of The Combination of Seluang Fish (Rasbora spp.) and Pasak Bumi (Eurycoma Longifolia Jack) on Systemic Inflammation and Neurotransmitter in Stunting Model Rat (Rattus Novergicus)
(1) Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia
(2) Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia
(3) Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia
(4) Brawijaya University, Malang, East Java, Indonesia
(5) Lambung Mangkurat University, Banjarmasin, South Kalimantan, Indonesia
(*) Corresponding Author
Abstract
Background: Stunting causes growth and development issues which are characterized by a systemic inflammatory response and neurotransmitter disorder. The South Kalimantan natural resources i.e. extracts of Pasak Bumi (Eurycoma longifolia Jack) and seluang fish have the potential as a modified therapy for stunting management.
Objective: To analyze the effect of Pasak Bumi and seluang fish administration in overcoming systemic inflammation and neurotransmitter disorders in the stunting rats model.
Methods: Rats were divided into six group i.e Positive Control (KP): Stunting rats + placebo + standard feed; (P1): stunting rats + 70% EPB 15 mg/kg body weight (BW) + standard feed; (P2): Stunting rats + DHA 1 mg/kg BW + standard feed; (P3): Stunting rats + EPB 15 mg/kg BW + DHA 1 mg/kg BW + standard feed; (P4): Stunting rats + seluang fish; (P5): Stunting rats + EPB 15 mg/kg BW + seluang fish for 5 weeks; Parameters measured were levels of IL-6, TNF-α, and serotonin.
Results: There were significant differences in levels of TNF-α (p = 0.000) and serotonin (p = 0.000).
Conclusion: The administration of Pasak Bumi extract and seluang fish was able to inhibit systemic inflammation and neurotransmitter dysregulation.Keywords
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Mutasa K, Tome J, Rukobo S, Govha M, Mushayanembwa P, Matimba FS, Chiorera CK, Majo FD, Tavengwa NV, Mutasa B, Chasekwa B. Stunting Status and Exposure to Infection and Inflammation in Early Life Shape Antibacterial Immune Cell Function Among Zimbabwean Children. Frontiers in immunology. 2022;13.899296. doi: 10.3389/fimmu.2022.899296.
Bourke CD, Berkley JA, Prendergast AJ. Immune dysfunction as a cause and consequence of malnutrition. Trends in immunology. 2016;37(6):386-398.
Brodin P, Davis MM. Human immune system variation. Nature reviews immunology. 2017;17(1):21-29.
Alaaraj N, Soliman A, Rogol AD. Growth of malnourished infants and children: how is inflammation involved?. Expert Review of Endocrinology & Metabolism. 2021;16(5): 213-216.
Amadi B, Zyambo K, Chandwe K, Besa E, Mulenga C, Mwakamui S, Siyumbwa S, Croft S, Banda R, Chipunza M, Chifunda K. Adaptation of the small intestine to microbial enteropathogens in Zambian children with stunting. Nature microbiology. 2021;6(4):445-454.
Wen B, Njunge JM, Bourdon C, Gonzales GB, Gichuki BM, Lee D, Wishart DS, Ngari M, Chimwezi E, Thitiri J, Mwalekwa L. Systemic inflammation and metabolic disturbances underlie inpatient mortality among ill children with severe malnutrition. Science advances. 2022;8(7):eabj6779. doi: 10.1126/sciadv.abj6779.
Syed S, Manji KP, McDonald CM, Kisenge R, Aboud S, Sudfeld C, Locks L, Liu E, Fawzi WW, Duggan CP. Biomarkers of systemic inflammation and growth in early infancy are associated with stunting in young Tanzanian children. Nutrients. 2018;10(9):1158.
Furman D, Campisi J, Verdin E, Carrera-Bastos P, Targ S, Franceschi C, Ferrucci L, Gilroy DW, Fasano A, Miller GW, Miller AH. Chronic inflammation in the etiology of disease across the life span. Nature medicine. 2019;25(12):1822-1832.
Mujico, JR. , Pérez-de-Heredia, F, Gómez-Martínez, S, Marcos, A. Malnutrition and Inflammation. In: Khatami, M, editor. Inflammation, Chronic Diseases and Cancer - Cell and Molecular Biology, Immunology and Clinical Bases [Internet]. London: IntechOpen; 2012. [cited 2022 Sep 21]. Available from: https://www.intechopen.com/chapters/31367 doi: 10.5772/25324.
Prendergast AJ, Rukobo S, Chasekwa B, Mutasa K, Ntozini R, Mbuya MN, Jones A, Moulton LH, Stoltzfus RJ, Humphrey JH. Stunting is characterized by chronic inflammation in Zimbabwean infants. PloS one. 2014;9(2):e86928. https://doi.org/10.1371/journal.pone.0086928
Sederquist B, Fernandez-Vojvodich P, Zaman F, Sävendahl L. Recent research on the growth plate: Impact of inflammatory cytokines on longitudinal bone growth. Journal of molecular endocrinology. 2014;53(1):T35-44.
Millward DJ. Nutrition, infection and stunting: the roles of deficiencies of individual nutrients and foods, and of inflammation, as determinants of reduced linear growth of children. Nutr Res Rev. 2017;30(1):50-72. doi: 10.1017/S0954422416000238.
Vindas MA, Johansen IB, Folkedal O, Höglund E, Gorissen M, Flik G, Kristiansen TS, Øverli Ø. Brain serotonergic activation in growth-stunted farmed salmon: adaption versus pathology. Royal Society open science. 2016 May 25;3(5):160030.
Shah R, Courtiol E, Castellanos FX, Teixeira CM. Abnormal serotonin levels during perinatal development lead to behavioral deficits in adulthood. Frontiers in behavioral neuroscience. 2018 Jun 6;12:114.
Sanyoto D, Triawanti, Noor M. The effect of ethanol extract of pasak bumi (Eurycoma longifolia Jack.) on neurogenesis and neuroinflammation of rat post protein malnutrition. IOP Conference Series: Earth and Environmental Science. 2021;913(1):012091. doi:10.1088/1755-1315/913/1/012091.
Wu H, Denna T, Storkersen J, Gerriets V. Beyond a neurotransmitter: The role of serotonin in inflammation and immunity. Pharmacol Res. 2019;140:100-114. doi:10.1016/j.phrs.2018.06.015
Haleem DJ, & Mahmood K. Brain serotonin in high-fat diet-induced weight gain, anxiety and spatial memory in rats. Nutritional neuroscience. 2021; 24(3), 226-235.
Triawanti T, Yunanto A, Dwi Sanyoto D, Wana Nuramin H. Nutritional Status Improvement in Malnourished Rat (Rattus norvegicus) after Seluang Fish (Rasbora spp.) Treatment. Current Research in Nutrition and Food Science Journal. 2018;6(1):127-134. doi:10.12944/crnfsj.6.1.14
Ruan J , Li Z , Zhang Y , Chen Y, Liu M , Han L , Zhang YI and Wang T. Bioactive Constituents from the Roots of Eurycoma longifolia. Molecules. 2019. 24(3157):1-16.
Calder PC. Eicosapentaenoic and docosahexaenoic acid derived specialised pro-resolving mediators: Concentrations in humans and the effects of age, sex, disease and increased omega-3 fatty acid intake. Biochimie. 2020 Nov 1;178:105-23.
Shibabaw T. Omega-3 polyunsaturated fatty acids: anti-inflammatory and anti-hypertriglyceridemia mechanisms in cardiovascular disease. Molecular and Cellular Biochemistry. 2021 Feb;476(2):993-1003.
Roe K. An inflammation classification system using cytokine parameters. Scand J Immunol. 2020;93(2). doi:10.1111/sji.12970
Manzella CR, Jayawardena D, Pagani W, Li Y, Alrefai WA, Bauer J, Jung B, Weber CR, Gill RK. Serum Serotonin Differentiates Between Disease Activity States in Crohn's Patients. Inflamm Bowel Dis. 2020 Sep 18;26(10):1607-1618. doi: 10.1093/ibd/izaa208.
Sanyoto DD, Noor MS, Triawanti T. Potential Combinations of Pasak Bumi (Eurycoma longifolia Jack), Docosahexaenoic Acid, and Seluang Fish (Rasbora spp.) to Improving Oxidative Stress of Rats (Rattus norvegicus) Brain Undernutrition. Open Access Macedonian Journal of Medical Sciences. 2022 Jan 1;10(A):25-32.
Triawanti, Sanyoto DD, Noor MS. The supplementation of pasak bumi (Eurycoma longifolia Jack.) in undernourished rats to increase spatial memory through antioxidant mechanism. Clinical Nutrition Experimental. 2020 Oct 1;33:49-59.
Abd El-Maksoud AM, Khairy SA, Sharada HM, Abdalla MS, Ahmed NF. Evaluation of pro-inflammatory cytokines in nutritionally stunted Egyptian children. Egyptian Pediatric Association Gazette. 2017 Sep 1;65(3):80-4.
Stephensen C. Primer on Immune Response and Interface with Malnutrition. Nutrition and Infectious Diseases. 2020:83-110. doi:10.1007/978-3-030-56913-6_3
Al-Khayri JM, Sahana GR, Nagella P, Joseph BV, Alessa FM, Al-Mssallem MQ. Flavonoids as Potential Anti-Inflammatory Molecules: A Review. Molecules. 2022 Jan;27(9):2901.
Kany S, Vollrath JT, Relja B. Cytokines in Inflammatory Disease. Int J Mol Sci. 2019 Nov 28;20(23):6008. doi: 10.3390/ijms20236008. PMID: 31795299.
Yao C, Narumiya S. Prostaglandin‐cytokine crosstalk in chronic inflammation. British journal of pharmacology. 2019 Feb;176(3):337-54.
Zelová H, Hošek J. TNF-α signalling and inflammation: interactions between old acquaintances. Inflamm Res. 2013;62(7):641-651. doi:10.1007/s00011-013-0633-0
Tanaka T, Narazaki M, Kishimoto T. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014;6(10):a016295. doi:10.1101/cshperspect.a016295
Lui JC, Nilsson O, Baron J. Recent research on the growth plate: Recent insights into the regulation of the growth plate. J Mol Endocrinol. 2014;53(1):T1-T9. doi:10.1530/JME-14-0022
Millward DJ. Knowledge gained from studies of leucine consumption in animals and humans. J Nutr. 2012;142(12):2212S-2219S. doi:10.3945/jn.111.157370
Wit JM, Camacho-Hübner C. Endocrine regulation of longitudinal bone growth. Endocr Dev. 2011;21:30-41. doi:10.1159/000328119
Zhang F, He Q, Tsang WP, Garvey WT, Chan WY, Wan C. Insulin exerts direct, IGF-1 independent actions in growth plate chondrocytes. Bone Res. 2014;2:14012. Published 2014 Jul 1. doi:10.1038/boneres.2014.12
Siddle K. Signalling by insulin and IGF receptors: supporting acts and new players. J Mol Endocrinol. 2011;47(1):R1-R10. Published 2011 Jun 17. doi:10.1530/JME-11-0022
Carter-Su C, Schwartz J, Argetsinger LS. Growth hormone signaling pathways. Growth Horm IGF Res. 2016;28:11-15. doi:10.1016/j.ghir.2015.09.00
Darvin P, Joung YH, Yang YM. JAK2-STAT5B pathway and osteoblast differentiation. JAKSTAT. 2013;2(4):e24931. doi:10.4161/jkst.24931
Fernandez-Vojvodich P, Zaman F, Sävendahl L. Interleukin-6 acts locally on the growth plate to impair bone growth. Ann Rheum Dis. 2013;72(10):e24. doi:10.1136/annrheumdis-2013-204112
Farquharson C, Ahmed SF. Inflammation and linear bone growth: the inhibitory role of SOCS2 on GH/IGF-1 signaling. Pediatric nephrology. 2013 Apr;28(4):547-56.
Shi C, Shi R, Guo H. Tumor necrosis factor α reduces gonadotropin-releasing hormone release through increase of forkhead box protein O1 activity. Neuroreport. 2020;31(6):473-477. doi:10.1097/WNR.0000000000001424
Chaingam J, Juengwatanatrakul T, Yusakul G, Kanchanapoom T, Putalun W. HPLC-UV-based simultaneous determination of Canthin-6-one alkaloids, quassinoids, and scopoletin: the active ingredients in Eurycoma Longifolia jack and Eurycoma Harmandiana pierre, and their anti-inflammatory activities. Journal of AOAC International. 2021 May;104(3):802-10.
Hien DT, Long TP, Thao TP, Lee JH, Trang DT, Minh NT, Van Cuong P, Dang NH, Dat NT. Anti-inflammatory effects of alkaloid enriched extract from roots of Eurycoma longifolia Jack. Asian Pacific Journal of Tropical Biomedicine. 2019 Jan 1;9(1):18.
Sheffler ZM, Reddy V, Pillarisetty LS. Physiology, Neurotransmitters. In: StatPearls. Treasure Island (FL): StatPearls Publishing; May 8, 2022.
Manuel-Apolinar L, Rocha L, Damasio L, Tesoro-Cruz E, Zarate A. Role of prenatal undernutrition in the expression of serotonin, dopamine and leptin receptors in adult mice: implications of food intake. Mol Med Rep. 2014;9(2):407-412. doi:10.3892/mmr.2013.1853
Mokler DJ, McGaughy JA, Bass D, et al. Prenatal Protein Malnutrition Leads to Hemispheric Differences in the Extracellular Concentrations of Norepinephrine, Dopamine and Serotonin in the Medial Prefrontal Cortex of Adult Rats. Front Neurosci. 2019;13:136. Published 2019 Mar 5. doi:10.3389/fnins.2019.00136
Hernández-Rodríguez J, Chagoya-Guzmán G, Mondragón-Herrera JA, Oca AB, Mercado-Camargo R, Manjarrez-Gutiérrez G. How intrauterine growth restriction due to nutritional stress changes the function of key proteins in brain serotonin metabolism during development. Cómo la restricción del crecimiento intrauterino debida al estrés nutricional cambia la función de proteínas clave en el metabolismo de la serotonina cerebral durante el desarrollo. Bol Med Hosp Infant Mex. 2021;78(6):571-583. doi:10.24875/BMHIM.20000334
Murnane KS. Serotonin 2A receptors are a stress response system: implications for post-traumatic stress disorder. Behav Pharmacol. 2019;30(2 and 3-Spec Issue):151-162. doi:10.1097/FBP.0000000000000459
Manjarrez-Gutiérrez G, Hernández-Rodríguez J, & Mondragón-Herrera JA. Nutritional Recovery and its Effect on Tryptophan-5-Hydroxylases Expression. Cell Number and on Changes Caused by Intrauterine Growth Restriction in the Developing Brain. J Nutr Food Sci. 2020; 10, 774
Höglund E, Øverli Ø, Winberg S. Tryptophan metabolic pathways and brain serotonergic activity: a comparative review. Frontiers in endocrinology. 2019:158.
Herr N, Bode C, Duerschmied D. The effects of serotonin in immune cells. Frontiers in cardiovascular medicine. 2017 Jul 20;4:48.
Yu B, Becnel J, Zerfaoui M, Rohatgi R, Boulares AH, Nichols CD. Serotonin 5-hydroxytryptamine(2A) receptor activation suppresses tumor necrosis factor-alpha-induced inflammation with extraordinary potency. J Pharmacol Exp Ther (2008) 327:316–23. 10.1124/jpet.108.143461
Patrick RP, Ames BN. Vitamin D and the omega-3 fatty acids control serotonin synthesis and action, part 2: relevance for ADHD, bipolar disorder, schizophrenia, and impulsive behavior. FASEB J. 2015;29(6):2207-2222. doi:10.1096/fj.14-268342
Günther J, Schulte K, Wenzel D, Malinowska B, Schlicker E. Prostaglandins of the E series inhibit monoamine release via EP3 receptors: proof with the competitive EP3 receptor antagonist L-826,266. Naunyn Schmiedebergs Arch Pharmacol. 2010;381(1):21-31. doi:10.1007/s00210-009-0478-9
Vedin I, Cederholm T, Freund-Levi Y, et al. Reduced prostaglandin F2 alpha release from blood mononuclear leukocytes after oral supplementation of omega3 fatty acids: the OmegAD study. J Lipid Res. 2010;51(5):1179-1185. doi:10.1194/jlr.M002667
Paila YD, Ganguly S, Chattopadhyay A. Metabolic depletion of sphingolipids impairs ligand binding and signaling of human serotonin1A receptors. Biochemistry. 2010;49(11):2389-2397. doi:10.1021/bi1001536
Cialdella-Kam L, Nieman DC, Knab AM, Shanely RA, Meaney MP, Jin F, Sha W, Ghosh S. A mixed Flavonoid-Fish oil supplement induces immune-enhancing and anti-inflammatory transcriptomic changes in adult obese and overweight women—A randomized controlled trial. Nutrients. 2016 May 11;8(5):277.
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DOI: https://doi.org/10.26714/magnamed.10.1.2023.27-39
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