Diabetic Wound Healing Activity of Anabas testudineus Fish Scales

Erwin Rosadi(1), Lia Yulia Budiarti(2), Bhisma Ridho Romadhon(3), Nida Nurkhalishah(4), Nur Salsabila Apriliani Risma Putri(5), Nur Azmina Aisyah(6), Isnaini Isnaini(7*)


(1) Faculty of Fishery and Marine University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
(2) Faculty of Medicine University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
(3) Faculty of Medicine University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
(4) Faculty of Medicine University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
(5) Faculty of Medicine University of Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
(6) Sepuluh Nopember Institute of Technology, Surabaya, East Java, Indonesia
(7) [Scopus ID: 57204801189] FK Universitas Lambung Mangkurat, Banjarmasin, South Kalimantan, Indonesia
(*) Corresponding Author

Abstract


Background:  Anabas testudineus fish scales are part of the fish that has not been used optimally. Fish scales contain chitin. Chitin which undergoes deacetylation, produces chitosan. Chitosan is an efficacious compound for antibacterial and diabetic wound healing.

Objective: This study aimed to extract chitosan from fish scales and test the diabetic wound healing activity of fish scale extract gel

Methods: Extraction of fish scales using 0.3 M NaOH, 0.55 M HCl, and 50% NaOH. FTIR will analyze the extraction results to see the functional groups of the extract. Activity test of fish scale extracts diabetic wound healing gel was carried out on Rattus novergicus Wistar strain for 14 days. There were five treatments: negative control, positive control given octenillinR, and fish scale extract gel conc. 5%, 10%, and 15%.

Results: The results showed that the fish scales extract contained chitosan, and gel extract fish scale conc 5% have diabetic wound healing activity better than 10% and 15%.

Conclusion: A. testudineus fish scale extract contains chitosan, as seen from the FTIR results.


Keywords


Chitosan; FTIR; Wound healing; Anabas Testudineus

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References


Al-Rasheed A, Handool KO, Garba B, Noordin MM, Bejo SK, Kamal FM, et al. (2018). Crude extracts of epi-dermal mucus and epidermis of climb-ing perch Anabas testudineus and its antibacterial and hemolytic activities. The Egyptian Journal of Aquatic Research, 44(2), 125–9.

Rosadi E, Buadiarti LY. (2020). Stock Assessment, Uji Aktivitas Antibakteri dan Wound Healing Sumberdaya Ikan Pepuyu (Anabas testudineus, Bloch 1792) di Kalimantan Selatan. Banjar-masin.

Gonzalez AC de O, Costa TF, An-drade Z de A, Medrado ARAP. (2016). Wound healing-A literature review. An Bras Dermatol, 91, 614–20.

Rosadi E, Budiarti LY, Zayanti NA, Isnaini. (2020). Antibacterial and wound healing activity of A. testudi-neus fish (Anabas Testudineus) mu-cus. Journal of Wetlands Environmental Management, 10(1), 12.

Irawan C, Nata IF, Putra MD, Marisa R, Asnia M, Arifin YF. (2018). Bi-opolymer of Chitosan from Fish Scales as Natural Coagulant for Iron–Contaminated Groundwater Treat-ment. Jurnal Rekayasa Kimia & Ling-kungan, 13(2), 93–9.

Matica MA, Aachmann FL, Tøndervik A, Sletta H, Ostafe V. (2019). Chi-tosan as a wound dressing starting ma-terial: Antimicrobial properties and mode of action. Int J Mol Sci, 20(23), 1–33.

Tsai HC, Sheng C, Chang LS, Wen ZH, Ho CY, Chen CM. (2021). Chi-tosan-microencapsulated rhEGF in promoting wound healing. J Wound Care, 110-121.

Djaenudin D, Budianto E, Saepudin E, Nasir M. (2019). Ekstraksi kitosan dari cangkang rajungan pada lama dan pengulangan perendaman yang ber-beda. Jurnal Teknologi Perikanan dan Kelautan, 10(1), 49–59.

Saputra, N. T., Suartha, I. N., & Dharmayudha, A. A. G. O. (2018). Agen diabetagonik streptozotocin un-tuk membuat tikus putih jantan diabe-tes mellitus. Buletin Veteriner Udaya-na, 116-121.

Rupina W, Trianto HF, Fitrianingrum I. (2016). Efek Salep Ekstrak Etanol 70% Daun Karamunting terhadap Re-epitelisasi Luka Insisi Kulit Tikus Wistar. eJournal Kedokteran Indonesia, 4(1), 26–30.

Izzati NH, Humaira N, Ni’mah L. (2018). Identifikasi awal pengaruh konsentrasi NaOH pada pembuatan kitosan dari limbah sisik ikan A. tes-tudineus. In: Prosiding Seminar Na-sional Industri Kimia dan Sumber Daya Alam 2018. p. 31–5.

Sukma, Mismawati A, Fajar Pamungkas B, Diachanty S, Zuraida I. (2022). Komposisi Proksimat dan pro-fil mineral tulang dan sisik ikan A. tes-tudineus (Anabas testudineus). Media Teknologi Hasil Perikanan, 10(3), 185–91. Available from: https://doi.org/10.35800/mthp.10.3.2022.36798

Hazmi AT, Ahmad FB, Akmal MHM, Ralib AAM, Ali FB. (2022). Fungal chitosan for potential application in piezoelectric energy harvesting: Re-view on experimental procedure of chitosan extraction. Alexandria Engi-neering Journal.

Ssekatawa K, Byarugaba DK, Wam-pande EM, Moja TN, Nxumalo E, Maaza M, et al. (2021). Isolation and characterization of chitosan from Ugandan edible mushrooms, Nile perch scales and banana weevils for biomedical applications. Sci Rep, 11(1).

Zeng X, Chen B, Wang L, Sun Y, Jin Z, Liu X, et al. (2023). Chi-tosan@Puerarin hydrogel for acceler-ated wound healing in diabetic sub-jects by miR-29ab1 mediated inflam-matory axis suppression. Bioact Mater, 19, 653–65.

Zahiruddin W, Ariesta A, Salamah E. (2008). Karakteristik mutu dan kelarutan kitosan dari ampas silase kepala udang windu (Penaeus mono-don). Buletin Teknologi Hasil Perikanan, 11(2), 140-151.

scales and banana weevils for biomed-ical applications. Sci Rep, 11(1).

Zeng X, Chen B, Wang L, Sun Y, Jin Z, Liu X, et al. (2023). Chi-tosan@Puerarin hydrogel for acceler-ated wound healing in diabetic sub-jects by miR-29ab1 mediated inflam-matory axis suppression. Bioact Mater, 19, 653–65.

Zahiruddin W, Ariesta A, Salamah E. (2008). Karakteristik mutu dan kelarutan kitosan dari ampas silase kepala udang windu (Penaeus mono-don). Buletin Teknologi Hasil Perikanan, 11(2), 140-151.

Ansel HC. Pengantar Bentuk Sediaan Farmasi, diterjemahkan oleh Ibrahim, F., (2005). Edisi IV. Penerbit UI Press. 605–619 p.

Shedoeva A, Leavesley D, Upton Z, Fan C. (2019). Wound healing and the use of medicinal plants. Evidence-based Complementary and Alternative Medicine: Hindawi Limited, 1-30.

Rohani S. Isolation and Characteriza-tion of Wound Healing Compounds from Chloroform Extract of Binahong Leaves (Anredera cordifolia (Ten.) Steenis). MAGNA MEDICA Berk Ilm Kedokt dan Kesehat. 2021;8(1):40.

Patel, S., Srivastava, S., Singh, M. R., & Singh, D. (2019). Mechanistic in-sight into diabetic wounds: Pathogen-esis, molecular targets and treatment strategies to pace wound healing. Bio-medicine & Pharmacothera-py, 112, 108615.


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