Isolation and Characterization of Wound Healing Compounds from Chloroform Extract of Binahong Leaves (Anredera cordifolia (Ten.) Steenis)

Siti Rohani(1*)


(1) FK UMPalembang
(*) Corresponding Author

Abstract


The leaves of Binahong (Anredera cordifolia (Ten) Steenis) have been widely studied to have benefits and uses in various medical purposes, one of which is in the wound healing process. The existence of several active compounds contained in the leaves of the Binahong tree plant have an important role in wound healing, such as flavonoids, terpenoids, alkaloids, saponins, ascorbic acid and citrate compounds, these compounds are the targeted for further process of this natural product. The eminence of the extraction method that is used repeatedly and consistently to obtain pure compounds from Binahong leaves to be isolated is the one of topics discussed in this literature study. The perspective of implementation from liquid-solid extraction followed by liquid-liquid extraction using chloroform as a solvent is discussed comprehensively in this article. The identification of the isolation of compounds are able to conducted by implemented a thin layer chromatography (TLC) techniques, and for the further structural determination by UV, MS, FTIR, NMR spectroscopy and separation techniques using GC-MS. Respectively, the components are successfully to be identified and reported in previous research, for the following criteria that have been completed, some isolated active compound are purposed as the candidate for the wound healing agent. For the further application it is reasonable to implied these matters for some pharmaceutical products.

Keywords


Anredera cordifolia (Ten) Steenis; Spectroscopy; UV; FTIR; GCMS

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References


Kusumanti, E. & Sugiharto, S. Effect of dietary supplementation of binahong leaf meal, betel nut meal or their combination on serum albumin and globulin, fecal endoparasites and bacterial counts in milk of Saanen goats suffering from subclinical mastitis. Agric. Nat. Resour. 51, 415–419 (2017).

Istyastono, E. & Yuliani, S. H. Scarless wound healing gel with Binahong (Anredera cordifolia (Ten) Steenis) leaves extract and celecoxib as the active ingredients. in Proceedings of the 1st International Conference on Science and Technology 2015 (ICST-2015) vol. 1755 160001 (2016).

Gizaw, M. et al. Electrospun Fibers as a Dressing Material for Drug and Biological Agent Delivery in Wound Healing Applications. Bioengineering vol. 5 (2018).

Nasir, A., Sign, W. & Test, R. The Influence of Binahong Leaves (Anredera Cordifolia) on Healing of Flour Albuson Female Students of Class XI SMAN 1 Paciran Lamongan District. J. Vocat. Nurs. 1, 80–85 (2020).

Gonzalez, A. C. de O., Costa, T. F., Andrade, Z. de A. & Medrado, A. R. A. P. Wound healing - A literature review. An. Bras. Dermatol. 91, 614–620 (2016).

Kintoko, K. et al. Effect of Diabetes Condition on Topical Treatment of Binahong Leaf Fraction in Wound Healing Process. Maj. Obat Tradis. 22, 103 (2017).

Hasiib, E., Riyanti, R. & Hartono, M. Pengaruh Pemberian Ekstrak Daun Binahong (Anredera Cordifolia (Ten.) Steenis) Dalam Air Minum Terhadap Performa Broiler. J. Ilm. Peternak. Terpadu 3, 233359 (2015).

Dong, S. et al. Antibacterial activity and mechanism of action saponins from Chenopodium quinoa Willd. husks against foodborne pathogenic bacteria. Ind. Crops Prod. 149, 112350 (2020).

Mahibalan, S., Stephen, M., Nethran, R. T., Khan, R. & Begum, S. Dermal wound healing potency of single alkaloid (betaine) versus standardized crude alkaloid enriched-ointment of Evolvulus alsinoides. Pharm. Biol. 54, 2851–2856 (2016).

Porras-Reyes, B. H., Lewis, W. H., Roman, J., Simchowitz, L. & Mustoe, T. A. Enhancement of wound healing by the alkaloid taspine defining mechanism of action. Proc. Soc. Exp. Biol. Med. Soc. Exp. Biol. Med. (New York, N.Y.) 203, 18–25 (1993).

Moores, J. Vitamin C: a wound healing perspective. Br. J. Community Nurs. Suppl, S6, S8-11 (2013).

Bikker, A., Wielders, J., van Loo, R. & Loubert, M. Ascorbic acid deficiency impairs wound healing in surgical patients: Four case reports. Int. J. Surg. Open 2, 15–18 (2016).

Nagoba, B. et al. Treatment of skin and soft tissue infections caused by Pseudomonas aeruginosa—A review of our experiences with citric acid over the past 20 years. Wound Med. 19, 5–9 (2017).

Nagoba, B. S., Aghav, V. V, Wadher, B. J., Rao, A. & Selkar, S. P. The use of citric Acid for the treatment of chronic non-healing sinus. Iran. J. Med. Sci. 36, 63–64 (2011).

Andrade-Eiroa, A., Canle, M., Leroy-Cancellieri, V. & Cerdà, V. Solid-phase extraction of organic compounds: A critical review. part ii. TrAC Trends Anal. Chem. 80, 655–667 (2016).

Zhang, Q.-W., Lin, L.-G. & Ye, W.-C. Techniques for extraction and isolation of natural products: a comprehensive review. Chin. Med. 13, 20 (2018).

Schoemig, V., Isik, E., Martin, L. & Berensmeier, S. Solid liquid liquid extraction of porcine gastric mucins from homogenized animal material. RSC Adv. 7, 39708–39717 (2017).

Vasilescu, M. N. & Medvedovici, A. V. Herbicides. in Encyclopedia of Analytical Science (Second Edition) (eds. Worsfold, P., Townshend, A. & Poole, C. B. T.-E. of A. S. (Second E.) 243–260 (Elsevier, 2005). doi:https://doi.org/10.1016/B0-12-369397-7/00256-9.

Berk, Z. Chapter 11 - Extraction. in Food Science and Technology (ed. Berk, Z. B. T.-F. P. E. and T.) 259–277 (Academic Press, 2009). doi:https://doi.org/10.1016/B978-0-12-373660-4.00011-9.

Hage, D. S. 1 - Chromatography. in Principles and Applications of Clinical Mass Spectrometry (eds. Rifai, N., Horvath, A. R. & Wittwer, C. T. B. T.-P. and A. of C. M. S.) 1–32 (Elsevier, 2018). doi:https://doi.org/10.1016/B978-0-12-816063-3.00001-3.

Kagarise, B. A. 3 - Peptide Mapping at the Nanomole Level. in (eds. LEFKOVITS, I. & PERNIS, B. B. T.-I. M.) 69–80 (Academic Press, 1979). doi:https://doi.org/10.1016/B978-0-12-442750-1.50008-5.

Ikan, R. & Crammer, B. Organic Chemistry, Compound Detection. in Encyclopedia of Physical Science and Technology (Third Edition) (ed. Meyers, R. A. B. T.-E. of P. S. and T. (Third E.) 459–496 (Academic Press, 2003). doi:https://doi.org/10.1016/B0-12-227410-5/00541-X.

Santiago, M. & Strobel, S. Chapter Twenty-Four - Thin Layer Chromatography. in Laboratory Methods in Enzymology: Cell, Lipid and Carbohydrate (ed. Lorsch, J. B. T.-M. in E.) vol. 533 303–324 (Academic Press, 2013).

Hostettmann, K. & Marston, A. Saponins. Encyclopedia of Analytical Science (Second Edition) 205–209 (2005) doi:https://doi.org/10.1016/B0-12-369397-7/00548-3.

Ismail, A. A., van de Voort, F. R. & Sedman, J. Chapter 4 Fourier transform infrared spectroscopy: Principles and applications. in Instrumental Methods in Food Analysis (eds. Paré, J. R. J. & Bélanger, J. M. R. B. T.-T. and I. in A. C.) vol. 18 93–139 (Elsevier, 1997).

Markovich, R. J. & Pidgeon, C. Introduction to Fourier Transform Infrared Spectroscopy and Applications in the Pharmaceutical Sciences. Pharm. Res. 8, 663–675 (1991).

Mohamed, M. A., Jaafar, J., Ismail, A. F., Othman, M. H. D. & Rahman, M. A. Chapter 1 - Fourier Transform Infrared (FTIR) Spectroscopy. in Membrane Characterization (eds. Hilal, N., Ismail, A. F., Matsuura, T. & Oatley-Radcliffe, D. B. T.-M. C.) 3–29 (Elsevier, 2017). doi:https://doi.org/10.1016/B978-0-444-63776-5.00001-2.

Pavia, D L., Lampman, G M., Kriz, G. S. Introduction to Spectroscopy. (1995). doi:10.1201/9781420050691.pt1.

Deda, O., Gika, H., Raikos, N. & Theodoridis, G. Chapter 4 - GC-MS-Based Metabolic Phenotyping. in The Handbook of Metabolic Phenotyping (eds. Lindon, J. C., Nicholson, J. K. & Holmes, E. B. T.-T. H. of M. P.) 137–169 (Elsevier, 2019). doi:https://doi.org/10.1016/B978-0-12-812293-8.00004-9.

Sparkman, O. D., Penton, Z. E. & Kitson, F. G. Chapter 1 - Introduction and History. in Gas Chromatography and Mass Spectrometry (Second edition) (eds. Sparkman, O. D., Penton, Z. E. & Kitson, F. G. B. T.-G. C. and M. S. (Second E.) 2–13 (Academic Press, 2011). doi:https://doi.org/10.1016/B978-0-12-373628-4.00001-0.

Gilbert, J. R. et al. Chapter 17 - Industrial Applications of High-Resolution GC/MS. in Advanced Techniques in Gas Chromatography–Mass Spectrometry (GC–MS–MS and GC–TOF–MS) for Environmental Chemistry (eds. Ferrer, I. & Thurman, E. M. B. T.-C. A. C.) vol. 61 403–429 (Elsevier, 2013).

Beira, M. et al. 1H NMR Relaxometry and Diffusometry Study of Magnetic and Nonmagnetic Ionic Liquid-Based Solutions: Cosolvent and Temperature Effects. J. Phys. Chem. B 121, 11472–11484 (2017).

Pauli, G. F., Jaki, B. U. & Lankin, D. C. Quantitative 1H NMR: Development and Potential of a Method for Natural Products Analysis. J. Nat. Prod. 68, 133–149 (2005).

Dais, P. & Spyros, A. Chapter 4 - Nuclear Magnetic Resonance. in Chemical Analysis of Food: Techniques and Applications (ed. Picó, Y. B. T.-C. A. of F. T. and A.) 91–115 (Academic Press, 2012). doi:https://doi.org/10.1016/B978-0-12-384862-8.00004-2.

Riegel, S. D. & Leskowitz, G. M. Benchtop NMR spectrometers in academic teaching. TrAC Trends Anal. Chem. 83, 27–38 (2016).

Hollmann, M. W., Durieux, M. E. & Fisher, D. M. Local Anesthetics and the Inflammatory Response: A New Therapeutic Indication? Anesthesiology 93, 858–875 (2000).

Cañedo-Dorantes, L. & Cañedo-Ayala, M. Skin acute wound healing: A comprehensive review. Int. J. Inflam. 2019, (2019).

Boyko, T. V, Longaker, M. T. & Yang, G. P. Review of the Current Management of Pressure Ulcers. Adv. wound care 7, 57–67 (2018).

Tandara, A. A. & Mustoe, T. A. Oxygen in wound healing--more than a nutrient. World J. Surg. 28, 294–300 (2004).

Nuutila, K., Katayama, S., Vuola, J. & Kankuri, E. Human Wound-Healing Research: Issues and Perspectives for Studies Using Wide-Scale Analytic Platforms. Adv. wound care 3, 264–271 (2014).

Lordan, R., Tsoupras, A. & Zabetakis, I. Chapter 2 - Inflammation. in The Impact of Nutrition and Statins on Cardiovascular Diseases (eds. Zabetakis, I., Lordan, R. & Tsoupras, A. B. T.-T. I. of N. and S. on C. D.) 23–51 (Academic Press, 2019). doi:https://doi.org/10.1016/B978-0-12-813792-5.00002-1.

García-Pérez, P., Barreal, M. E., Rojo-De Dios, L., Cameselle-Teijeiro, J. F. & Gallego, P. P. Chapter 2 - Bioactive Natural Products From the Genus Kalanchoe as Cancer Chemopreventive Agents: A Review. Stud. Nat. Prod. Chem. 61, 49–84 (2019).

Kumar, V., Abbas, A., Fausto, N. & Aster, J. Pathologic Basis of Disease, Professional Edition. (Elsevier, 2009).

Eming, S. A., Martin, P. & Tomic-Canic, M. Wound repair and regeneration: mechanisms, signaling, and translation. Sci. Transl. Med. 6, 265sr6-265sr6 (2014).

Mulyata, S. Analisis imunohistokimia TGFβ indikasi hambatan kesembuhan luka operasi episiotomi pada tikus Sprague Dawley. Indones. Symp. Obstet. Anesth. (2002).

Li, W. et al. Wound-healing perspectives. Dermatol. Clin. 23, 181–192 (2005).

Perencanaan Buku Media Informasi tentang Manfaat Tanaman Binahong untuk Terapi Herbal pada Penyembuh Luka. (Universitas Komputer Indonesia).

Manoi, F. & Balittro. Binahong (Anredera cordifolia) sebagai obat. J. War. Penelit. dan Pengemb. Tanam. Ind. 15, 3–4 (2009).

Pengaruh Konsentrasi Ekstrak Daun Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Jumlah Pembuluh Darah pada Penyembuhan Luka Gingiva Tikus Putih (Rattus norvegicus). (Universitas Gajah Mada).

Hartono, E. ., Sugeng, S. . & Puradisastra, S. Efek Ekstrak Etanol Daun Binahong (Anredera cordifolia (Ten.) Steenis) dalam Mempercepat Durasi Penyembuhan Luka Sayat pada Mencit Swiss Webster Jantan. (Universitas Kristen Maranatha, 2011).

Paju, N., Yamlean, P. V. . & Kojong, N. Uji Efektivitas Salep Ekstrak Daun Binahong (Anredera Cordifolia (Ten.) Steenis) pada Kelinci Oryctolagus cuniculus) yang Terinfeksi Bakteri Staphylococcus aureus. J. Ilm. Farm. UNSRAT 2, 2302 – 2493 (2013).

Darsana, I. G. ., Besung, I. N. . & Mahatmi, H. Potensi daun binahong (Anredera cordifolia (Tenore) Steenis) dalam menghambat pertumbuhan bakteri Escherichia coli secara in vitro. Indones. Med. Veterinus 1, 337 – 351 (2012).

Robbins, S. . & Kumar, V. Buku Ajar Patologi. (EGC, 2007).

Guyton & Hall. Buku Ajar Fisiologi Kedokteran Ed.9. (EGC, 1997).

Nijveldt, R. J. et al. Flavonoids: a review of probable mechanisms of action and potential applications. Am. J. Clin. Nutr. 74, 418–425 (2001).

Selawa, W., Runtuwene, M. R. . & Citraningtyas, G. Kandungan Flavonoid dan Kapasitas Antioksidan Total Ekstrak Etanol Daun Binahong [Anredera cordifolia (Ten) Steenis]. J. Ilm. Farasi UNSRAT. Manad. 18–22 (2013).

Hasanoglu, A. et al. Efficacy of Micronized Flavonoid Fraction in Healing of Clean and Infected Wounds. Int. J. Angiol. Off. Publ. Int. Coll. Angiol. Inc 10, 41–44 (2001).

Kumar, S. & Pandey, A. K. Chemistry and Biological Activities of Flavonoids: An Overview. Sci. World J. 2013, 162750 (2013).

Harbone, J. . Penentuan cara modern menganalisis tumbuhan. (Institut Teknologi Bandung, 1987).

Nirmala, W., Budiyanto, E., Ardi, Y.W., Hendry, S. Pemanfaatan Ekstrak Daun Kemangi (Ocinum canum) Sebagai Permen Herbal Pencegah Bau Mulut. (UNY, 2009).

Yang, R.-Y., Lin, S. & Kuo, G. Content and distribution of flavonoids among 91 edible plant species. Asia Pac. J. Clin. Nutr. 17 Suppl 1, 275–279 (2008).

Bhagwat, S. & Haytowitz, D. B. USDA Database for the Flavonoid Content of Selected Foods. (2015).

Susmayanti, W. Isolasi, Identifikasi dan Uji Toksisitas Senyawa FlavonoidDari Ekstrak Daun Binahong (Anredera cordiforlia (Tenns) Stennis). (Universitas Diponegoro, 2011).

Djamil, R., Wahyudi, P., Wahono, S. & Hanafi. Antioxidant Activity of Flavonoid from Anredera Cordifolia (Ten) steenis Leaves. J. Pharm. (2012).

Mahindru, S. & Resident, J. Evaluation of effect of topical application of 3% citric acid on wound healing. Int. J. Curr. Res. Med. Sci. 3, 30–38 (2017).

Mahre, M. et al. Haematological changes and wound healing effects of sildenafil citrate in diabetic albino rats. Sokoto J. Vet. Sci. 15, (2017).


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DOI: https://doi.org/10.26714/magnamed.8.1.2021.40-59

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