The Cytoxic Effects Of Forest Honey (Apis dorsata) On T47D Breast Cancer Cells

Malik Hisyam Adnan(1*), Maya Dian Rakhmawatie(2), Yanuarita Tursinawati(3)


(1) Faculty of Medicine, Universitas Muhammadiyah Semarang, Semarang, Central Java, Indonesia
(2) Faculty of Medicine, Universitas Muhammadiyah Semarang, Semarang, Central Java, Indonesia
(3) Faculty of Medicine, Universitas Muhammadiyah Semarang, Semarang, Central Java, Indonesia
(*) Corresponding Author

Abstract


Background: In 2018, an estimated 2 million people had breast cancer. Forest honey (Apis dorsata) can have antioxidant activity due to the presence of flavonoid saponin, alkaloid, and tannin compound, therefore can be used as anticancer through the induction of apoptosis.

Objective: To determine the IC50 of forest honey (Apis dorsata) on T47D breast cancer cells and see the morphology of T47D cells after administration of forest honey.

Methods: This study is an in vitro test of the cytotoxic activity of forest honey against T47D breast cancer cells using the MTT assay method. The concentration of forest honey was prepared by the two-fold microdilution method in the range of 1000 - 31.25 µg/mL. Doxorubicin was used as a control drug with a concentration of 20 - 0.675 µg/mL. The morphology of T47D cells after treatment was observed with an inverted microscope with 400x magnification.

Results: Forest honey (Apis dorsata) from any concentration did not show any inhibition of growth of T47D breast cancer cells. Meanwhile, doxorubicin had an IC50 of 3.746 µg/mL. The morphology of T47D cells with honey administration showed many live cells with formazan crystals.

Conclusion: Forest honey has no cytotoxic activity against T47D breast cancer cells.


Keywords


Honey; IC50; breast cancer; doxorubicin; apoptosis

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References


Kementerian Kesehatan. Peraturan Menteri Kesehatan Republik Indonesia Nomor 34 Tahun 2015 tentang Penanggulangan Kanker Payudara dan Kanker Leher Rahim. Indonesia; 2015 p. 3.

Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, Piñeros M, et al. Estimating the global cancer incidence and mortality in 2018. Vol. 144, International Journal of Cancer. Wiley-Liss Inc.; 2019. p. 1941–53.

Kemenkes. Hari Kanker Sedunia 2019 [Internet]. 2019 [cited 2021 Mar 11]. Available from: https://www.kemkes.go.id/article/view/19020100003/hari-kanker-sedunia-2019.html

Panigoro S, Hernowo BS, Handojo, Purwanto H, Haryono SJ, Arif W, et al. Panduan penatalaksanaan kanker payudara. 2019.

Condorelli R, Vaz-Luis I. Managing side effects in adjuvant endocrine therapy for breast cancer. Vol. 18, Expert Review of Anticancer Therapy. Taylor and Francis Ltd; 2018. p. 1101–12.

Wein L, Loi S. Mechanisms of resistance of chemotherapy in early-stage triple negative breast cancer. Breast. 2017 Aug 1;34:S27–30.

Waheed M, Hussain MB, Javed A, Mushtaq Z, Hassan S, Shariati MA, et al. Honey and cancer: A mechanistic review. Vol. 38, Clinical Nutrition. Churchill Livingstone; 2019. p. 2499–503.

Yaacob NS, Nengsih A, Norazmi MN. Tualang honey promotes apoptotic cell death induced by tamoxifen in breast cancer cell lines. Evidence-based Complement Altern Med. 2013;2013.

Aryappalli P, Al-Qubaisi SS, Attoub S, George JA, Arafat K, Ramadi KB, et al. The IL-6/STAT3 Signaling Pathway Is an Early Target of Manuka Honey-Induced Suppression of Human Breast Cancer Cells. Front Oncol. 2017 Aug 14;7(AUG).

Johan van Meerloo, Gertjan J.L. Kaspers and JC. Cancer Cell Culture: Methods and Protocols. Vol. 731, Springer Science. 2003. 237–245 p.

Riss TL, Moravec RA, Niles AL, Duellman S, Benink HA, Worzella TJ, et al. Cell Viability Assays. Assay Guid Man. 2016 Jul 1;

Thorn CF, Oshiro C, Marsh S, Hernandez-Boussard T, McLeod H, Klein TE, et al. Doxorubicin pathways: Pharmacodynamics and adverse effects. Pharmacogenet Genomics. 2011;21(7):440–6.

Asensio-López MC, Soler F, Pascual-Figal D, Fernández-Belda F, Lax A. Doxorubicin-induced oxidative stress: The protective effect of nicorandil on HL-1 cardiomyocytes. PLoS One. 2017 Feb 1;12(2).

Hou Y, Zhou M, Xie J, Chao P, Feng Q, Wu J. High glucose levels promote the proliferation of breast cancer cells through GTPases. Breast Cancer Targets Ther. 2017;9:429–36.

Nakagawa T, Lanaspa MA, Millan IS, Fini M, Rivard CJ, Sanchez-Lozada LG, et al. Fructose contributes to the Warburg effect for cancer growth. Cancer Metab. 2020;8(1):1–12.

Fan X, Liu H, Liu M, Wang Y, Qiu L, Cui Y. Increased utilization of fructose has a positive effect on the development of breast cancer. PeerJ. 2017;2017(9).

Han J, Zhang L, Guo H, Wysham WZ, Roque DR, Willson AK, et al. Glucose promotes cell proliferation, glucose uptake and invasion in endometrial cancer cells via AMPK/mTOR/S6 and MAPK signaling. Gynecol Oncol. 2015 Sep 1;138(3):668.

Gautam G. General principles of MTT assay method requirements : procedure of MTT assay on HeK cells to check cell viability : 2018.

Rasyid R, Sriwahyuni F, Rivai H. Mechanism of death of T47D breast cancer cells against dichloromethane fractions from Kandis cortex (Garcinia cowa roxb). WORLD J Pharm Pharm Sci. 2019;8(8):174–83.

Saputri DS, Putri YE. Aktivitas Antioksidan Madu Hutan Di Beberapa Kecamatan Di Kabupaten Sumbawa Besar. J TAMBORA. 2017;2(3):1–6.


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DOI: https://doi.org/10.26714/magnamed.11.2.2024.181-188

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