Efek Pemberian Ekstrak Buah Kersen ( Muntingia Calabura) terhadap Histopatologi Paru Tikus yang Dipapar Asap Rokok

Ika Dyah Kurniati(1*), Dyah Mustika Nugraheni(2)


(1) FK UNIMUS, Semarang
(2) FK UNIMUS, Semarang
(*) Corresponding Author

Abstract


Latar belakang: Oksidan yang terkandung dalam asap rokok dapat menyebabkan terjadinya stres oksidatif. Buah kersen (Muntingia calabura) mengandung antioksidan diantaranya flavonoid. Penelitian ini bertujuan mengetahui efek proteksi ekstrak buah kersen terhadap kerusakan histologis alveolus paru tikus Sprague dawley yang terpapar asap rokok
Metode: Tikus sejumlah dibagi 4 kelompok, kemudian di papar asap rokok 7 batang perhari dan ekstrak buah kersen (EBK) selama 4 minggu. Kelompok pertama sebagai kontrol (K1) diberi plasebo, kelompok kedua (K2) dipapar asap rokok saja, sedangkan sisanya merupakan kelompok Perlakuan 1 (P1) diberi EBK 100mg/kgBB/hari dan Perlakuan 2 (P2) diberi EBK 200mg/kgBB/hari. Perbedaan derajat kerusakan alveolus di analisis dengan Kruskal-Wallis Test.
Hasil: Rerata derajat kerusakan alveolus pada kelompok kontrol (K1)sejumlah (69,44) kelompok perlakuan 1 (P1) sejumlah (80,00) dan kelompok perlakuan 2 (P2) sejumlah (79,63) sedangkan yang terendah yaitu pada kelompok kontrol paparan asap rokok (K2) sejumlah (61,11). Nilai P =  0.464, menunjukkan bahwa tidak terdapat perbedaaan yang bermakna derajat kerusakan alveolus antar kelompok, dimana rerata derajat kerusakan alveolus paru > 60%  (kerusakan berat).
Simpulan : Pemberian ekstrak buah kersen tidak berpengaruh terhadap gambaran mikroskopis paru tikus yang di papar asap rokok.

 Background: Oxidant which is contain in cigarette smoke increases oxidative stress. Kersen fruit (Muntingia calabura) contains antioxidants including flavonoids. The purpose of this study is to proves protecting effect of kersen fruit extract on the lung tissue.Method: Rats were divided into 4 groups that had been exposed 7 cigarrete smoke a day and given kersen fruit extract (KFE) for 4 weeks. The first group as the control group (K) was given a placebo, second group were exposed cigarrete smoke only, treatment group 1 (T1) and treatment group 2 (T2) were treated with 100 and 200mg/kgBodyWeight/ day KFE, respectively. The difference in the degree of alveolar damages were analyzed.Results: There were no significant differences in the degree of alveolar damages between groups. The average degree of alveolar damages was severe, where the alveolar damages were> 60%.Conclusion: Kersen fruit has no effect on the histopathology of alveolar damage 

Keywords


asap rokok; buah kersen; kerusakan histologi; alveolus paru

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DOI: https://doi.org/10.26714/magnamed.1.5.2018.68-76

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