Aktivitas Antifungi Ekstrak Metanol Bunga Kamboja Putih (Plumeria acuminata) Terhadap Aspergillus clavatus

Berty Oktavia(1*)


(1) Universitas Tanjungpura Pontianak
(*) Corresponding Author

Abstract


Aspergillus clavatus is able to produce mycotoxin compound that cause pathology in animals and humans. The growth of A. clavatus can be inhibited by using methanol extract of Plumeria acuminata Ait. since P. acuminata has an ability to produce natural antifungal. This study aims to investigate the concentrate of methanol extract in P. acuminata which can be utilized to inhibit the growth of specific mushroom, particularly A. clavatus AMB1. This experiment is conducted for three months, January to March 2017 in Microbiology Laboratory of Mathematics and Natural Sciences of Tanjungpura University Pontianak and in Laboratory of Plantation Product Processing of State Polytechnic Pontianak. This study employed Completely Randomized Design (CRD) with 7 treatments, specifically negative control (0 g/ml), solvent control (tween 80), positif control (ketoconazole 0,02 g/ml) and concentrate of P. acuminata (0,6 g/ml, 0,7 g/ml, 0,8 g/ml, 0,9 g/ml). The findings indicate that the lowest concentrate of extract, particularly 0,6 g/ml has 57,6% ability to inhibit the growth of A. clavatus AMB1 and strong level of antifungal activity. Extract concentrate of 0,9 g/ml is the highest extract concentrate that has similar antifungal activity to 0,02 g/ml  antifungal ketoconazole and extremely strong level of antifungal activity.


Keywords


Aspergillus clavatus AMB1, natural antifungal, Plumeria acuminata

Full Text:

PDF

References


Aulifa, DL, Aryantha, INP, & Sukrasno, 2014, ’Aktivitas antijamur ekstrak metanol dari tumbuhan rempah-rempahan’ Jurnal Ilmu-Ilmu Hayati dan Fisik’ vol. 16, no. 1, hal.10-15

Botha, JC, Legg, JM, Truter, M & Sulyok, M, 2014, ‘Multitoxin analysis of Aspergillus clavatus infected feed samples implicated in two outbreaks of Neuromycotoxicosis in Cattle in South Africa’, Onderstepoort Journal of Veterinary Research, vol.81, no.1, hal.1-6

Cahyani, NF & Suhartanti, D, 2015, ‘Aktivitas antifungi ekstrak etanol 70% campuran rimpang Curcuma domestica dengan biji Phaleria marcocarpa terhadap jamur Trametes sp. sebagai sumber belajar siswa SMA Kelas X’, Jupemasi-Pbio, vol.1, no.2, hal:256-262

Falahati, M, Ghojoghi A, Abastabar, M, Ghasemi, Z, Farahyar S, Roudbary, M, Hedayati, TM, Armaki, TM & Hoseinnejad, A, 2016, ‘The first case of total dystrophic onychomicosis caused by Aspergillus clavatus resistant to antifungal drugs’, Mycopathologia, vol.181, no.3, hal.273-277

Fitriani, S, Raharjo & Trimulyono, G, 2013,’Aktivitas antifungi ekstrak daun kedondong (spondias pinnata) dalam menghambat pertumbuhan aspergillus niger’ LenteraBio, vol. 2, no. 2, hal. 125-129

Gunawan, PW, D, Ningsih & M, Aprilia, 2010, ’Aktivitas antibakteri dan penyembuhan luka fraksi-fraksi ekstrak etanol daun kamboja (Plumeria acuminate Ait.) pada kulit kelinci yang diinfeksi Staphylococcus aureus’, Jurnal Farmasi Indonesia, vol.7, no.2, hal.73-77

Gupta M, Mazumder UK, Gomathi P, & Selvan VT, 2006, ‘Antiinflammatory evaluation of leaves of Plumeria acuminata’, BMC Complem. and Alter.Med., vol.6, hal.36-42

Gupta M, Mazumder UK, Gomathi P, & Selvan VT ,2008,‘Antimicrobal activity of methanol extracts of Plumeriaacuminate Ait. leaves and Tephrosia purpurea (Linn.) Pers. roots’, Journal Natural Product Radiance, vol.72, no.2, hal.102-105

Harborne, JB, 1987, Metode Fitokimia : penentuan cara modern menganalisis tumbuhan, Edisi Kedua, Penerjemah : Kosaih Padmawinata dan Iwang Soediro, ITB, Bandung

Hopkins,WG, 1999, Introduction to plant physiology, 2nd Ed, John Wiley and Sons Inc, New York

Lopez-Diaz, T & Flannigan, B, 1997, ‘Production of patulin and cytochalasin e by Aspergillus clavatus during malting of barley and wheat’, Int. J. Food Microbiol, vol.35, hal:129-136.

Muhtadi, Ambarwati, R & Yuliani, R, 2012,’Aktivitas antibakteri ekstrak etanol dan fraksi kulit batang belimbing wuluh (Averrhoa bilimbi Linn.) terhadap bakteri Klebsiella pneumonia dan Staphylococcus epidermis beserta bioautografinya’, Biomedika, vol. 4, no.2, hal.1-9

Novriyanti, E, Santosa, E, Syafii, W, Turjaman, M & Sitepu, IR, 2010, ‘Antifungal Activity of Wood Extract of Aquilaria crassna Pierre ex Lecomte Against Agarwood-Inducing Fungi, Fusarium solani’, Journal od Forestry Research, vol. 7, no. 2, hal.155-165

Patron, DD, 2006, ‘Aspergillus, Health Implication and Recommendations for Public Health Food Safety’, Internet Journal of Food Safety, vol.8, hal.19-23

Philips, RM & Rosen, T, 2002, Topical antifungal agents, In: Wolverton ES, editor, Comprehensive Dermatology Drug Therapy, Indianapolis, WB Saunders Company, Indiana

Rahman, ZA, 2011,’Uji banding efektivitas Allium sativa (bawang putih) 2% dengan ketokonazol 2% secara in vitro terhadap pertumbuhan Malassezia furfur pada Pityriasis versicolor’ Jurnal Ilmu-Ilmu Kesehatan Surya Medika, vol. 7, no. 1, hal.21-26

Rai, IGA, 2006, ‘Aktivitas fungisida ekstrak daun saba (Piper majusculum Blume) terhadap jamur Fusarium oxysporum f. sp. vanillae penyebab penyakit busuk batang pada panili’, Tesis, Program Magister Program Studi Bioteknologi, Universitas Udayana

Rochani, N, 2009, ‘Uji aktivitas antijamur ekstrak daun binahong (Anrederacordifolia (Tenore) Steen) terhadap Candida albicans serta skrining fitokimianya’,Skripsi, Universitas Muhammadiyah Surakarta, Surakarta

Salni, Aminasih, N & Sriviona, R, 2013, ‘Isolasi senyawa antijamur dari rimpang lengkuas putih (Alpinia galangal (L.) Wild) dan penentuan konsentrasi hambat minimum terhadap Candida albicans’, Prosiding Semirata FMIPA Universitas Lampung

Samson RA, J, Houbraken, U, Thrane, JC, Frisvad & B, Anderson, 2010, Food and Indoor Fungi, Fungal Biodiversity Centre Utrecht, Neterlands

Sulistyawati, D & Mulyati, S, 2009, ‘Uji aktivitas antifungi infusa daun jambu mete (Anacardium occidentale L) terhadap Candida albicans, Jurnal Biomedika, vol. 2, no.1, hal.47-51

Tiwari, Kumar, Kaur, M, Kaur, G & Kaur H, 2011, ‘Phytochemical screening and extraction a review’, Internationale Pharmaceutica Sciencia, vol. 1, issue 1

Varga, J, Due, M, Frisvad, JC & Samson, RA, 2007, ‘Taxonomic revision of Aspergillus section Clavati based on molecular, Morphological and Physiological Data’. Stud. Mycol, vol. 59, hal.89-106

Villanueva, MJ, Arugay, VAM, & Ramos, HZR, 2008, ‘In vitro antimycotic activity of four medicinal plants versus clotrimazole in the treatment of otomycosis: a preliminary study’, Philippine Journal of Otolaryngology Head and Neck Surgery, vol. 23, no.1, hal.5-8

Wahyuni, S, Mukarlina & Yanti, HA, 2014,’Aktivitas Antifungi Ekstrak Metanol Daun Buas-Buas (Premna serratifolia) Terhadap Jamur Diplodia sp. Pada Jeruk Siam (Citrus nobilis var. microcarpa)’ Protobiont, vol. 3, no. 2, hal.274-279

Warsinah, Kusumawati, E, & Sunarto, 2011,‘Identifikasi senyawa antifungi dari kulit batang kecapi (Sandoricum koetjape) dan aktivitasnya terhadap Candida albicans’, Majalah Obat Tradisional, vol. 16, no. 3, hal.165-173


Article Metrics

Abstract view : 1234 times
PDF - 145 times

DOI: https://doi.org/10.26714/jlabmed.1.2.2017.22-29

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Jurnal Labora Medika



 

View My Stats
Program Studi Teknologi Laboratorium Medik Universitas Muhammadiyah Semarang

Jl. Kedungmundu Raya No. 18 Semarang Gedung Laboratorium Kesehatan Universitas Muhammadiyah Semarang

Phone: 02476740295
Email: [email protected]