Kadar Hb dan Perubahan Berat Badan Tikus Wistar Model Anemia Defisiensi Besi Setelah Pemberian Minyak Ikan Patin

Abstract

Anemia is a health problem with little progress in its management, even though
the global prevalence of anemia is relatively high. Anemia due to iron deficiency affects
weight gain in children. Giving catfish oil to anemic rats was done to see the effect on
Hb levels and body weight.
This research is a true experiment with a randomized pre-post-test design with
a control group. Male Wistar rats were divided into four groups, namely K- (rats fed
standard), K+ (rats fed standard without iron), P1 (rats fed standard without iron,
getting catfish oil 0.039 ml/day), P2 (rats fed standard without iron, supplemented with
ferrous sulfate 0.126 mg/day) for 14 days. Hb levels were measured before and after
treatment using the ELISA method. The rats were weighed every day.
Paired t-test was conducted to determine differences in Hb levels and changes
in body weight before and after the intervention. In addition, the Anova test followed
by Post Hoc Bonferroni was carried out to analyze the difference in effect on the four
groups.
There were significant differences in body weight changes before treatment and after
treatment (p=0.00, p<0.05) on K-(13 gram ± 0.81), K+(5.5 gram ± 1.04), P1 (12 grams
± 0.7), P2 (13 grams ± 0.63). In addition, the study showed a significant increase in
Hb levels before and after treatment (p=0.00, p<0.05) at P1(4.7 g/dL ± 0.76) and
P2(5.13 g/dL ± 0.15 ), as well as a decrease in K- (0.09 g/dL ± 0.19) and K+ (0.37
g/dL ± 0.18).
There was an effect of giving catfish oil on Hb levels and changes in body
weight, which increased significantly in iron deficiency anemia Wistar rats.

Keyword: body weight, catfish oil, Hb levels iron deficiency anemia