Skrining Fitokimia, Total Fenol, dan Aktivitas Antioksidan Ekstrak Metanol Tangkai Sisir Buah Pisang Agung

Ade Wahyu Pratama(1*), Sri Rahayu Lestari(2), Abdul Gofur(3), Yunita Rakhmawati(4)


(1) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Indonesia
(2) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Indonesia
(3) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Indonesia
(4) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Indonesia
(*) Corresponding Author

Abstract


Free radicals have an unpaired electron in the outermost orbital causing degenerative diseases such as cancer. However, it can be
inhibited by antioxidants. Natural antioxidants are considered safer than synthetic antioxidants, which have harmful side effects
if consumed by the body in the long term. Natural antioxidants are obtained from natural ingredients such as plants. Agung
banana is one of the plants that have the potential to produce antioxidants. The community widely consumes these bananas,
increasing the waste of banana bunches stem. Banana bunch stem is one of the wastes that has not been widely used. This study
aims to determine secondary metabolites' content through phytochemical screening, total phenol, and antioxidant activity.
Phytochemical screening is a qualitative descriptive test with indicators of color change and the presence of precipitate. The screening
results showed that the agung banana bunch stem extract was positive for flavonoids, triterpene terpenoids, polyphenols, tannins,
and alkaloids. The total phenol test showed a result of 40,00 mg/g. The antioxidant activity test showed an IC50 value of 190,55
µg/ml, which was classified as weak. Research shows that the relatively small relationship between total phenol causes low/weak
antioxidant activity.


Keywords


Phenolic Total, Antioxidant Activity, Agung Banana, Bunch Stem

Full Text:

PDF

References


Achakzai, A. K. K., Achakzai, P., Masood, A.,

Kayani, S. A., dan Bakhsh Tareen, R.

(2009). Response Of Plant Parts And Age

On The Distribution Of Secondary

Metabolites On Plants Found In Quetta.

Pak. J. Bot, 41(5), 2129–2135.

Anliza, S., dan Hamtini. (2017). Uji Aktivitas

Antioksidan Ekstrak Metanol Dari Daun

Alocasia Macrorrhizos Dengan Metode

DPPH. Jurnal Medikes, 4(1), 101–106.

Arifin, S., Damanhuri, dan Soetopo, L. (2013).

Gucialit Kabupaten Lumajang

Observation And Characterization Of

Banana ( Musa Spp ) In Gucialit Sub

District Lumajang Regency. Jurnal Produksi

Tanaman, 3(6), 480–486.

Badan Pusat Statistik. (2021). Produksi Buahbuahan.

Burda, dan Oleszek. (2001). Antioxidant and

antiradical activities of flavonoid. J. Agric.,

(6), 2774–2779.

Fukumoto, L. R., dan Mazza, G. (2000).

Assessing antioxidant and prooxidant

activities of phenolic compounds. Journal

of agricultural and food chemistry. Journal

of Agricultural and Food Chemistry. , 48(8),

–3604.

Ghasemzadeh, A., Jaafar, H. Z., dan Rahmat, A.

(2010). Antioxidant activities, total

phenolics and flavonoids content in two

varieties of Malaysia young ginger

(Zingiber officinale Roscoe). Molecules,

(6), 4324–4333.

Hasma, dan Winda. (2019). . Idengtifikasi

Senyawa Metabolit Sekunder Ekstrak

Kulit Pisang Kepok (Musa paradisiaca L)

Dengan Metode KLT. Jurnal Kesehatan

Menarang, 5(2), 125–131.

Huang, D. , O. B., dan Prior, R. L. (2005). The

Chemistry Behind Antioxidant Capacity

Assays. Journal of Agricultural and Food

Chemistry, 53(6), 1841-1856.

Khadijah, K., Jayali, A. M., Umar, S., dan

Sasmita, I. (2017). Penentuan total fenolik

dan aktivitas antioksidan ekstrak etanolik

daun samama (Anthocephalus

macrophylus) asal Ternate, Maluku Utara.

. Jurnal Kimia Mulawarman, 15(1), 11–18.

Liu, Y., Liu, C., dan Li, J. (2020). Comparison of

vitamin c and its derivative antioxidant

activity: Evaluated by using density

functional theory. ACS Omega, 25467–

https://doi.org/10.1021/acsomega.0c043

Ncube, N. S., Afolayan, A. J., dan Okoh, A. I.

(2008). Assessment Techniques of

Antimicrobial Properties of Natural

Compounds of Plant Origin: Current

Methods and Future Trends. African

Journal of Biotechnology, 7(12), 1797–1806.

Nurhayati, N., Maryanto, M., dan Tafrikhah, R.

(2016). Ekstraksi Pektin dari Kulit dan

Tandan Pisang dengan Variasi Suhu dan

Metode (Pectin Extraction from Banana

Peels and Bunch with Various

Temperatures and Methods). Jurnal

Agritech, 36(03), 327.

https://doi.org/10.22146/agritech.16605

Prahardini, P. E. R., Yuniarti, N., dan

Krismawati, A. (2016). Karakterisasi

Varietas Unggul Pisang Mas Kirana dan

Agung Semeru di Kabupaten Lumajang.

Buletin Plasma Nutfah, 16(2), 126.

https://doi.org/10.21082/blpn.v16n2.20

p126-133

Pratama, A. N., dan Busman, H. (2020). Potential

of Soybean Antioxidant (Glycine Max L) on

Capturing Free Radicals. 11(1), 497–504.

https://doi.org/10.35816/jiskh.v10i2.333

Rakhmawati, Y., Lestari, S. R., Pratama, A. W.,

dan Sulistiyowati, N. (2022). Nutrition Facts

Analysis of Agung Banana Flour During

Ripening.

Rakhmawati, Y., dan Rahayu Lestari, S. (2021).

Typical Characteristics of Agung Banana

(Musa paradisiaca) from Lumajang. KnE

Life Sciences.

https://doi.org/10.18502/kls.v0i0.8893

Sayuti, K., dan Yenrina, R. (2015).

ANTIOKSIDAN ALAMI dan

SINTETIK. Andalas University Press.

Sayuti, M. (2017). Pengaruh perbedaan metode

ekstraksi, bagian dan jenis pelarut terhadap

rendemen dan aktivitas antioksidan

bambu laut (Isis hippuris). . Technology

Science and Engineering Journal, 1(3), 166–

Sirait, M. (2007). Penuntun Fitokimia dalam

Farmasi. ITB Press.

Soeksmanto, A., Hapsari, Y., dan Simanjuntak,

P. (2007). Kandungan Antioksidan pada

Beberapa Bagian Tanaman Mahkota

Dewa, Phaleria macrocarpa (Scheff)

Boerl.(Thymelaceae). Biodiversitas, 8(2),

–95.

Suretno, A. D., dan Adriyani, F. Y. (2020).

Limbah Agroindustri Pengemasan Pisang

Mas Kirana (Musa acumunata) Sebagai

Pakan Kambing di Lampung. In Seminar

Nasional Lahan Suboptimal, 686-694. 28.

Susanti, A, 686–684.

Susanti, A. D., Ardiana, D., dan Gumelar, P. G.

(2012). Polaritas pelarut sebagai pertimbangan

dalam pemilihan pelarut untuk ekstraksi

minyak bekatul dari bekatul varietas ketan

(Oriza sativa glatinosa).

Tetti, M. (2014). Ekstraksi, Pemisahan Senyawa,

dan Identifikasi Senyawa Aktif. . Jurnal

Kesehatan, 7(2).

Tristantini, D., Ismawati, A., Tegar Pradana, B.,

dan Gabriel Jonathan, J. (2016). Pengujian

Aktivitas Antioksidan Menggunakan

Metode DPPH pada Daun Tanjung

(Mimusops elengi L). Seminar Nasional

Teknik Kimia Kejuangan, 1–7.

Tsuda, Y. (2014). Isolation of Natural

Products. Japan Analytical Industry.


Article Metrics

Abstract view : 373 times
PDF - 165 times

DOI: https://doi.org/10.26714/jpg.12.2.2022.14%20-%2021

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Jurnal Pangan dan Gizi


Jurnal Pangan dan Gizi 
ISSN (print) 2086-6429 | 2086-6429  (online)
Organized by Department of Food Science and Technology, Universitas Muhammadiyah Semarang, Semarang, Indonesia
Published by Prodi Teknologi Pangan Universitas Muhammadiyah Semarang
W : https://jurnal.unimus.ac.id/index.php/JPDG 
E : [email protected], [email protected]

Jumlah Pengunjung :

 View My Stats

 

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.