Skrining Fitokimia, Total Fenol, dan Aktivitas Antioksidan Ekstrak Metanol Tangkai Sisir Buah Pisang Agung

Ade Wahyu Pratama(1*), Sri Rahayu Lestari(2), Abdul Gofur(3), Yunita Rakhmawati(4)


(1) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Indonesia
(2) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Indonesia
(3) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Indonesia
(4) Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Malang, Jl. Semarang No. 5 Malang, Indonesia
(*) Corresponding Author

Abstract


Free radicals have an unpaired electron in the outermost orbital causing degenerative diseases such as cancer. However, it can be
inhibited by antioxidants. Natural antioxidants are considered safer than synthetic antioxidants, which have harmful side effects
if consumed by the body in the long term. Natural antioxidants are obtained from natural ingredients such as plants. Agung
banana is one of the plants that have the potential to produce antioxidants. The community widely consumes these bananas,
increasing the waste of banana bunches stem. Banana bunch stem is one of the wastes that has not been widely used. This study
aims to determine secondary metabolites' content through phytochemical screening, total phenol, and antioxidant activity.
Phytochemical screening is a qualitative descriptive test with indicators of color change and the presence of precipitate. The screening
results showed that the agung banana bunch stem extract was positive for flavonoids, triterpene terpenoids, polyphenols, tannins,
and alkaloids. The total phenol test showed a result of 40,00 mg/g. The antioxidant activity test showed an IC50 value of 190,55
µg/ml, which was classified as weak. Research shows that the relatively small relationship between total phenol causes low/weak
antioxidant activity.


Keywords


Phenolic Total, Antioxidant Activity, Agung Banana, Bunch Stem

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